High-Plex Spatial Profiling
of Suspension Cells
SmartBioSurface® enables reliable, high-plex spatial profiling of suspension cells and supports integrated analysis of blood-derived and tissue samples in a unified workflow. The technology provides stable cell immobilization and is fully compatible with leading cyclic immunofluorescence platforms, including Bruker CellScape™, CellScape XR™, Quanterix Spatial PhenoCycler®, and Bio-Techne COMET™. This allows multiple rounds of staining, washing, and imaging while maintaining high image quality and assay reproducibility.
Key findings include:
- Excellent cell retention, with approximately 98.5% retention after 10 imaging cycles.
- Preservation of cell integrity throughout repeated staining and imaging procedures.
- Generation of robust, reproducible, and high-quality multiplex spatial data.
- Successful compatibility with major high-plex imaging platforms without significant cell loss.
By supporting validated tissue-based reagent panels, imaging chemistries, and analytical workflows, SmartBioSurface® enables researchers to extend established spatial biology methods to suspension cells without modifying existing protocols.
Overall, SmartBioSurface® is a robust, scalable, and reproducible solution for next-generation spatial biology, enabling direct comparison between tissue sections and suspension cell populations and providing deeper insights into complex biological systems. This integrated approach supports advanced applications in immuno-oncology, immunology, infectious diseases, liquid biopsy, and translational research.
